Histones are highly basic proteins involved in packaging DNA into chromatin, and histone modifications are fundamental in epigenetic regulation in eukaryotes. Among the numerous chromatin modifiers identified in Arabidopsis (Arabidopsis thaliana), MORF-RELATED GENE (MRG)1 and MRG2 have redundant functions in reading histone H3 lysine 36 trimethylation (H3K36me3). Here, we show that MRG2 binds histone chaperones belonging to the NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) family, including NAP1-RELATED PROTEIN (NRP)1 and NRP2. Characterization of the loss-of-function mutants mrg1 mrg2, nrp1 nrp2 and mrg1 mrg2 nrp1 nrp2 revealed that MRG1/MRG2 and NRP1/NRP2 regulate flowering time through fine-tuning transcription of floral genes by distinct molecular mechanisms. In particular, the physical interaction between NRP1/NRP2 and MRG1/MRG2 inhibited the binding of MRG1/MRG2 to the transcription factor CONSTANS (CO), leading to a transcriptional repression of FLOWERING LOCUS T (FT) through impeded H4K5 acetylation (H4K5ac) within the FT chromatin. By contrast, NRP1/NRP2 and MRG1/MRG2 act together, likely in a multiprotein complex manner, in promoting the transcription of FLOWERING LOCUS C (FLC) via an increase of both H4K5ac and H3K9ac in the FLC chromatin. Because the expression pattern of FLC represents the major category of differentially expressed genes identified by genome-wide RNA-sequencing analysis in the mrg1 mrg2, nrp1 nrp2 and mrg1 mrg2 nrp1 nrp2 mutants, it is reasonable to speculate that the NRP1/NRP2-MRG1/MRG2 complex may be involved in transcriptional activation of genes beyond FLC and flowering time control. © 2020 Society for Experimental Biology and John Wiley & Sons Ltd.Aortic regurgitation (AR) with intimal intussusception, secondary to aortic dissection, is relatively rare and the images are interesting findings. We report a typical case of severe AR with intimal intussusception, secondary to DeBakey type I aortic dissection, detected by contrast-enhanced computed tomography (CECT) and transesophageal echocardiography (TEE). Since there are three types of aortic regurgitation with aortic dissection, it is imperative to consider the most appropriate intervention for AR. The combination of CECT, TEE, and surgical findings may play an important role in determining the optimum surgical procedure for AR with aortic dissection. © 2020 Wiley Periodicals, Inc.Three new compounds containing a heptadentate lanthanide (LnIII) ion chelator functionalized with oligothiophenes, nThept(COOH)4 ( n = 1 , 2, or 3 ), were isolated. Their LnIII complexes not only display the characteristic metal-centred emission in the visible or near-infrared (NIR) but also generate singlet oxygen (1O2). Luminescence efficiencies (ϕLn) for [Eu 1Thept(COO)4]- and [Eu 2Thept(COO)4]- are ϕEu = 3% and 0.5% in TRIS buffer and 33% and 3% in 95% ethanol, respectively. 3Thept(COO)44- does not sensitize EuIII emission due to its low-lying triplet state. NIR luminescence is observed for all NIR-emitting LnIII and ligands with efficiencies of ϕYb = 0.002%, 0.005% and 0.04% for [Yb nThept(COO)4]- ( n = 1, 2, or 3), and ϕNd = 0.0007%, 0.002% and 0.02% for [Nd nThept(COO)4]- ( n = 1, 2, or 3) in TRIS buffer. In 95% ethanol, quantum yields of NIR luminescence increase and are ϕYb = 0.5%, 0.31% and 0.05% for [Yb nThept(COO)4]- ( n = 1, 2, or 3 ), and ϕNd = 0.40%, 0.45% and 0.12% for [Nd nThept(COO)4]- ( n = 1 , 2 , or 3 ). All complexes are capable of generating 1O2 in 95% ethanol with ϕ1Ο2 efficiencies which range from 2% to 29%. These complexes are toxic to HeLa cells when irradiated with UV light (λexc = 365 nm) for 2 minutes. IC50 values for the LnIII complexes are in the range 15.2 – 16.2 μM; the most potent compound is [Nd 2Thept(COO)4]-. The cell death mechanisms are further explored using an Annexin V – propidium iodide assay which suggests that cell death occurs through both apoptosis and necrosis. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.”To many, the design of medical curricula appears the reverse of common sense», Hatala and colleagues claimed with reference to an education that starts with the basic sciences and only at the very end presents students with signs and symptoms of actual patients. Indeed, students “may well discover that their knowledge is organized inadequately”. This article is protected by copyright. All rights reserved.BACKGROUND Organised inpatient (stroke unit) care is provided by multi-disciplinary teams that manage stroke patients. selleck chemicals llc This can been provided in a ward dedicated to stroke patients (stroke ward), with a peripatetic stroke team (mobile stroke team), or within a generic disability service (mixed rehabilitation ward). Team members aim to provide co-ordinated multi-disciplinary care using standard approaches to manage common post-stroke problems. OBJECTIVES • To assess the effects of organised inpatient (stroke unit) care compared with an alternative service. • To use a network meta-analysis (NMA) approach to assess different types of organised inpatient (stroke unit) care for people admitted to hospital after a stroke (the standard comparator was care in a general ward). Originally, we conducted this systematic review to clarify • The characteristic features of organised inpatient (stroke unit) care? • Whether organised inpatient (stroke unit) care provide better patient outcomes than alternative forms of care? oderate-quality evidence that stroke patients who receive organised inpatient (stroke unit) care are more likely to be alive, independent, and living at home one year after the stroke. The apparent benefits were independent of patient age, sex, initial stroke severity, or stroke type, and were most obvious in units based in a discrete stroke ward. We observed no systematic increase in the length of inpatient stay, but these findings had considerable uncertainty. TRIAL REGISTRATION ClinicalTrials.gov NCT00544622 NCT00843765. Copyright © 2020 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze-thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p  less then  .05). The addition of 250 µg/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 µg/ml (p  less then  .05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H.