Polymerase chain reaction (PCR)-based detection assays for Streptococcus equi subspecies equi often overestimate the prevalence of samples containing viable organisms. The objective of this study was to determine if viability could be determined using genome quantitation and detection of messenger RNA (mRNA) transcripts for the SeM gene of S. equi in pre- and post-cultured samples. Nasal secretions collected from 42 horses with suspected strangles were tested by culture and by quantitative PCR (qPCR) before and 24 hours after a culture step. Viable S. equi was determined based on the detection of S. equi via culture, the detection of mRNA transcripts for the SeM gene of S. equi by qPCR, and/or an increase in absolute number of SeM target genes of S. equi between pre- and post-cultured samples. Viability was determined in 28/42 samples based on isolation of S. equi (11 samples), the presence of mRNA transcripts for the SeM gene of S. equi (25), and/or an increase in absolute quantitation of the SeM gene of S. equi between pre- and post-culture (17). The overall agreement between culture alone and the three criteria to determine viability was 59%. The overall agreement for the detection of mRNA transcripts and increase in absolute target genes was 88% and 74%, respectively. The combination of mRNA transcripts and increase in absolute target genes was able to determine the viability status in all 42 samples. In the absence of a culture-positive result for S. equi, the determination of viability can be achieved by using molecular strategies applied to samples undergoing a 24-hour culture step.The physiological role of L-carnitine in equine species is worthy of investigation; however, the systemic content of free L-carnitine and its dynamic change in growing foals as well as in exercising horses are still poorly investigated. In this study, the influence of age and exercise on free serum L-carnitine levels was evaluated in equine species. Ten foals were monitored from 6 up to 18 months of age (group 1), whereas 60 horses were divided in six groups in accordance with their age group 2, 2-year-old; group 3, 3-year-old; group 4, 4-year-old; group 5, 5-year-old; group 6, 6-year-old; group 7, 7-year-old. To assess the age and sex effect on free serum L-carnitine values, blood samples were collected from foals and horses. Adult horses (groups 2-7) were subjected to a simulate 1,660-m race, and blood samples were collected before the simulate race (TPRE), within 10 minutes (TPOST10) from the end of race, and after 30 minutes (TPOST30) from the end of race. The amino acid levels were influenced by age (P less then .0001) in foals and horses. Decreased levels of amino acid were observed at TPOST10 with respect to TPRE and TPOST30. (P less then .001). The findings suggest that the biosynthetic pathway of L-carnitine is organizing and adapting to the metabolic needs of skeletal and cardiac muscle tissue in the course of growth. L-carnitine could play a role for the provision of energy to the exercising muscles. Further studies are needed to evaluate possible beneficial effects of L-carnitine during growing phase and on parameters of equine physical performance.Many studies on human intestinal microbiota indicate that gender difference is one of the key factors influencing microbial community composition. To date, the degree of influence that gender has on equid intestinal microbiota has not been reported. Thus, microbiota was analyzed in feces of seven female Przewalski’s horses (FRPHs) and seven male Przewalski’s horses (MRPHs) in this study, determining which microbiota characteristics respond to gender biases. The microbial community composition and structure were explored by 16S rRNA sequencing, followed by diversity analysis and difference analysis. Female Przewalski’s horses showed higher Shannon diversity than MRPHs, no difference in Simpson diversity, and displayed difference in beta diversity. Although gender had little effect on the overall microbiota, it significantly changed the dominant microbial community in each classification level. Male Przewalski’s horses contained significantly higher amounts of microorganisms related with diseases, including spirochetes (phylum), deltaproteobacteria (class), fibrobacteria (class), spirochaetia (class), desulfovibrionales (order), fibrobacterales (order), spirochaetales (order), and spirochaetaceae (family). Female Przewalski’s horses showed less than MRPHs in the top 10 genera. To our knowledge, this study is the first to document the gender-related intestinal microbiota profile in equines and discovered notable differences between the gender, which suggests that gender should be considered as a biological variable in future microbiota studies.The aim of the study was to assess the expression of the immune checkpoint inhibitor programmed death-ligand 1 (PD-L1) in equine sarcoids (ES). Programmed death-ligand 1 is expressed by various cancer cells to block T cell-mediated elimination of tumor cells. Antibodies targeting human PD-L1 were tested by immunohistochemistry for their cross-reactivity with equine PD-L1 using formalin-fixed, paraffin-embedded tissues. Ro-3306 datasheet Our results do not support an important role of PD-L1-mediated immune evasion in ES disease and hence do not offer a rationale for anti-PD-1/PD-L1-based immunotherapy against ES.The objective of this study was to quantify serum progesterone levels, uterine features, and pregnancy rates in acyclic, embryo recipient mares using a bovine progesterone-releasing intravaginal device in a commercial embryo transfer (ET) program. The study included 73 recipient mares of unknown breed, aged 3-10 years, weighing 350-500 kg, and kept under an intensive management system on Tifton 85 (Cynodon spp.) pastures with water and mineral salt ad libitum. The horses were divided into two groups a group with a progesterone-releasing intravaginal device (1 g progesterone, G-IVP4, n = 24) and a control group (G-iP4, n = 49) receiving an injection of 1,500 mg long-acting progesterone. Jugular blood was collected for the G-IVP4 group for subsequent progesterone measurement by radioimmunoassay on three occasions Day 0 (D0), intravaginal device was placed; Day 5 (D5), day of the ET; and Day 9 (D9), day of pregnancy diagnosis. There was an increase (P .05), with rates of 83.33% and 73.46% for G-IVP4 and G-iP4, respectively.