The established norms of Healthy Fitness Measurement Scale (HFMS V1.0) for Chinese urban elderly provide evaluation criteria for Chinese elderly healthy fitness level and facilitate exploration of healthy fitness status and its influencing factors in Chinese urban elderly.

To explore the feasibility of three-dimensional (3D) vessel wall imaging of carotid atherosclerotic plaques in ApoE

mice using 7.0T magnetic resonance imaging (MRI) with delays alternating with nutations for tailored excitation (DANTE)-prepared fast low-angle shot (DANTE-FLASH) technique.

Numerical simulations were performed for optimizing imaging parameters to maximize the wall-lumen contrast. NBQX cell line Six ApoE

and three wild-type mice were scanned using a 7.0T MRI scanner with DANTE-FLASH and multi-slice 2D RARE coupled with inflow outflow saturation bands (2D-IOSBRARE). The wall signal-to-noise ratio (SNRwall), lumen SNR (SNRlumen), wall-lumen contrast-to-noise ratio (CNR), lumen area (LA), and wall area (WA) were compared between DANTE- FLASH and 2D-IOSB-RARE sequences. Linear regression analysis was performed to assess the correlation between the MRI measurements and histopathological measurements of LA and WA.

Based on the simulation results, a flip angle of 15° and a train length of 150 were implemented in the live imaging study. Compared with 2D-IOSB-RARE, DANTE-FLASH provided a slightly reduced CNR (

< 0.001) but much improved slice resolution. The LA and WA measurements from the DANTE-FLASH and 2D-IOSB- RARE showed excellent agreement based on ICC analysis (LA ICC=0.94,

< 0.001; WA ICC=0.93,

< 0.001) and Bland-Altman plots. Strong correlations were observed between the MRI and histopathological measurements for both LA (

< 0.0001) and WA (

< 0.0001).

As a 3D black-blood MR sequence, DANTE-FLASH provides isotropic high spatial resolution to allow reliable visualization and quantitative evaluation of the arteriosclerotic lesions within the carotid artery of ApoE

mice using a 7.0T MRI scanner.

As a 3D black-blood MR sequence, DANTE-FLASH provides isotropic high spatial resolution to allow reliable visualization and quantitative evaluation of the arteriosclerotic lesions within the carotid artery of ApoE-/- mice using a 7.0T MRI scanner.

To determine whether miR-600 suppresses the proliferation of HeLa cells by inhibiting hypoxia-inducible factor-1

(HIF-1

) signaling pathway and its effect on expressions of cyclin D1 and vascular endothelial growth factor (VEGF).

HeLa cells were transfected with miR-600 mimic and plasmid-HIF-1

, either alone or in combination, to up-regulate miR-600 and HIF-1

expressions in the cells. Six hours after the transfection, the cell viability was assessed using MTT assay, and the mRNA and protein expressions of VEGF, cyclin D1, and HIF-1

were analyzed with qPCR and Western blotting.

The viability of HeLa cells showed no obvious changes 6 h after transfection with miR-600 mimic or Plasmid-HIF-1

. At 24 h and 48 h, the cells transfected with miR-600 mimic showed a time-dependent reduction of cell viability, while the cells transfected with Plasmid-HIF-1

alone and with both miR-600 mimic and Plasmid-HIF-1

showed increased cell viability. The cell viabilities in Plasmid-HIF-1

group were significantly higher than those in miR-600 mimic+Plasmid-HIF-1

group at 24 h and 48 h. Six hours after transfection with miR-600 mimic, the cells exhibited significantly decreased expressions of VEGF, cyclin D1, and HIF-1

, which were all significantly up-regulated in Plasmid-HIF-1

group and miR-600 mimic+Plasmid-HIF-1

group. VEGF, cyclin D1, and HIF-1

expressions were significant higher in Plasmid-HIF-1

group than in miR-600 mimic+ Plasmid-HIF-1

group.

miR-600 suppresses the proliferation of HeLa cells and down-regulate the expressions of cyclin D1 and VEGF by inhibiting HIF-1

signaling pathway.

miR-600 suppresses the proliferation of HeLa cells and down-regulate the expressions of cyclin D1 and VEGF by inhibiting HIF-1α signaling pathway.

To explore the molecular mechanism of

for improving injury of human aortic endothelial progenitor cells (HAECs).

Serum samples were collected from male SD rats treated with

(

=8) or saline (

= 5). HAECs cultured in normoxia or hypoxic condition (2% O

) were treated with serum from normal rats or with diluted serum (1% and 10%) from rats treated with Fuxinfang. The differentially expressed genes (DEGs) between

-treated and control cells were detected using high-throughput sequencing to screen the target DEGs that participated in arterial endothelial cell injury and underwent changes in response to both hypoxia and

treatment. AmiGo and String databases were used to infer the interactions among the target genes, and the expressions of the genes were analyzed in HAECs with different treatments using enzyme-linked immunosorbent assay (ELISA) and Western blotting.

HAECs cultured in hypoxia did not show obvious changes in cell morphology or expressions of hypoxia-related factors in response to trit NR4A1 expression and suppressing hypoxia-induced p38 phosphorylation.

The serum from Fuxinfang-treated rats can concentration-dependently inhibit the expressions of the DEGs occurring in hypoxia. Fuxinfang improves hypoxic injuries of HAECs possibly by down-regulating the expression of c-Fos to inhibit NR4A1 expression and suppressing hypoxia-induced p38 phosphorylation.

To investigate the effect of long non-coding RNA UPK1A-AS1 on glycolysis of hepatocellular carcinoma cells and its underlying molecular mechanisms.

A hepatocellular carcinoma (HCC) cell line with lentivirus-mediated stable UPK1A-AS1 overexpression and the cells infected with a negative control lentiviral vector were cultured under normoxic (21% O

) or hypoxic (1% O

) conditions for 24 h. The effect of UPK1A-AS1 overexpression on glycolysis of the HCC cells was examined. The expressions of glycolysis-related genes HIF1A, GLUT1, HK1, HK2 and PGK1 were detected by qRTPCR, and the effect of UPK1A-AS1 overexpression on HRE activity was detected by dual luciferase report assay. The HCC cells were treated with cycloheximide to detect the effect of UPK1A-AS1 overexpression on the stability of HIF-1

protein. Immunoprecipitation assay was used to analyze the changes in ubiquitin modification of HIF-1

protein in response to UPK1A-AS1 overexpression. The effects of UPK1A-AS1 overexpression and RNA interference of HIF-1

expression on glucose consumption, lactate production and expressions of HRE activity and glycolysis-related genes (HK1, HK2 and PGK1) were examined in the HCC cells.