brain water content no different than if NS had been given in place of mannitol. Only when the NSUO replacement ratio was 13, brain water was similar to that of control animals receiving mannitol alone. The recommendation to replace UO 11 with an equal volume of isotonic crystalloid following perioperative mannitol administration must recognize how this strategy could elevate brain water content compared to less vigorous replacement of UO.

In rats, NS replacement of UO 11 following mannitol administration leads to brain water content no different than if NS had been given in place of mannitol. Only when the NSUO replacement ratio was 13, brain water was similar to that of control animals receiving mannitol alone. The recommendation to replace UO 11 with an equal volume of isotonic crystalloid following perioperative mannitol administration must recognize how this strategy could elevate brain water content compared to less vigorous replacement of UO.Forkhead box protein O6 (FOXO6) has been recently identified as a novel regulator of oxidative stress in multiple pathological processes. However, whether FOXO6 participates in the regulation of oxidative stress of myocardial infarction is unclear. The present study was performed to evaluate the potential role of FOXO6 in regulating hypoxia-induced apoptosis and oxidative stress in cardiomyocytes in vitro. Our results demonstrated that FOXO6 expression was highly elevated in cardiomyocytes exposed to hypoxia. Downregulation of FOXO6 expression by the siRNA-mediated gene knockdown in hypoxia-exposed cardiomyocytes increased cell viability, while repressing apoptosis and reactive oxygen species (ROS) production. In contrast, overexpression of FOXO6 enhanced the sensitivity of cardiomyocytes to hypoxia-induced injury. Further, in-depth research revealed that knockdown of FOXO6 promoted the expression of sirtuin6 (SIRT6) and enhanced the activation of nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant signaling. Moreover, SIRT6 inhibition markedly blocked the FOXO6 knockdown-induced promotion effect on Nrf2 activation. In addition, Nrf2 inhibition partially reversed the FOXO6 knockdown-mediated protective effect against hypoxia-induced cardiomyocyte injury. Taken together, the findings of our study demonstrate that knockdown of FOXO6 is capable of protecting cardiomyocytes from hypoxia-induced apoptosis and oxidative stress by enhancing Nrf2 activation via upregulation of SIRT6. Our study highlights a potential role of FOXO6 in myocardial infarction and suggests it as an attractive target for cardioprotection.Despite adherence to treatment, individuals living with HIV have an increased risk for developing cognitive impairments, referred to as HIV-associated neurological disorders (HAND). Due to continued growth in the HIV population, particularly amongst the aging cohort, the neurobiological mechanisms of HAND are increasingly relevant. Similar to other viral proteins (e.g. PT2977 Tat, Gp120, Vpr), the Negative Factor (Nef) is associated with numerous adverse effects in the CNS as well as cognitive impairments. In particular, emerging data indicate the consequences of Nef may be facilitated by the modulation of cellular autophagy as well as its inclusion into extracellular vesicles (EVs). The present review examines evidence for the molecular mechanisms by which Nef might contribute to neuronal dysfunction underlying HAND, with a specific focus on autophagy and EVs. Based on the these data, we propose an integrated model by which Nef may contribute to underlying neuronal dysfunction in HAND and highlight potentially novel therapeutic targets for HAND. Graphical abstract.Teen dating violence is a complex issue associated with several deleterious consequences. Previous studies emphasize the importance of considering the heterogeneity of teen dating violence experiences to better understand this issue and its correlates. In this perspective, the present study aimed to identify gender-specific patterns of teen dating violence in heterosexual relationships based on directionality (victimization and perpetration) and forms of violence (psychological, physical and sexual). In addition, this study aimed to investigate how these patterns are differentially associated with attachment insecurities and emotion dysregulation. A total of 3100 adolescents who reported being in a heterosexual romantic relationship (mean age = 15.92 years; 60% girls) completed questionnaires on teen dating violence, romantic attachment and emotion dysregulation. Latent class analyses revealed four distinct patterns of teen dating violence. The first three patterns, namely Low dating violence (40% of girls and 54% of boys), Mutual psychological dating violence (34% of girls and 33% of boys) and Mutual psychological and physical dating violence (14% of girls and 5% of boys), were found for both genders. The last pattern differed greatly based on gender and was labeled Mutual psychological dating violence and sexual victimization in girls (12%) and Multiple dating violence victimization in boys (8%). Higher levels of emotion dysregulation and attachment insecurities were found in adolescents experiencing more complex patterns of dating violence. This study contributes to the development of teen dating violence prevention and intervention programs by identifying gender-specific patterns of teen dating violence and documenting their associations with important trauma-informed correlates.Youth initiated mentoring is a hybrid approach that empowers youth to identify and recruit natural mentors, potentially combining the strengths of informal mentoring relationships with the infrastructure and support provided by formal mentoring programs. This meta-analytic review examined the association between youth-initiated programs and youth outcomes across four domains academic and vocational functioning, social-emotional development, physical health, and psychosocial problems. Results indicated that youth-initiated programs are significantly associated with positive youth outcomes. There was a small-to-medium effect size of g = 0.30 for youth-initiated programs overall, which was based on 14 studies with 11 independent samples (3594 youth and 169 effect sizes) from 2006 to 2019. The effect size was somewhat larger (g = 0.40) when controlling for possible selection bias, and was moderated by participant gender and year of publication. Implications for theory and practice regarding this relatively new approach to mentoring are discussed.