Lastly, future perspectives and challenges remaining for systems metabolic engineering of E. coli are discussed.

Eprinomectin is used as an active pharmaceutical ingredient (API) in various drug products by the animal health industry. Several major related impurities of Eprinomectin API are not separated and coelute by the current United States Pharmacopeia method for Eprinomectin.

To develop and validate a true and reliable stability-indicating reversed phase HPLC method for assay and determination of related substances of Eprinomectin in bulk batches of Eprinomectin API.

HPLC analysis is carried out using a Kinetex-C8 column (100 mm × 4.6 mm i.d., 2.6 μm particle size) maintained at 30 °C with water-acetonitrile-isopropanol (48 + 42+10, v/v/v) as mobile phase-A and 100% acetonitrile as mobile phase-B. Analytes are separated by gradient elution at a flow rate of 0.7 mL/min and detected by UV at 252 nm. Total run time of the method is 30 min. Eprinomectin assay and estimation of all Eprinomectin related substances are obtained in a single HPLC run.

The HPLC method was able to separate all analytes of interest by gradient elution. The new method was successfully validated according to current ICH/VICH guidelines and was found to be specific, linear, accurate, precise, robust, and sensitive.

An HPLC method for assay of Eprinomectin and estimation of its related substances was successfully developed, validated, and demonstrated to be accurate, robust, specific, and stability-indicating.

The HPLC method presented in this paper is more desirable and suitable for routine analysis of bulk batches of Eprinomectin API and stability samples in quality control (QC) laboratories.

The HPLC method presented in this paper is more desirable and suitable for routine analysis of bulk batches of Eprinomectin API and stability samples in quality control (QC) laboratories.

Standard-of-care for opioid use disorder (OUD) includes medication and counseling. BMS309403 mw However, there is an unmet need for complementary approaches to treat OUD patients coping with pain; furthermore, few studies have probed neurobiological features of pain or its management during OUD treatment. This preliminary study examines neurobiological and behavioral effects of a virtual reality-based meditative intervention in patients undergoing methadone maintenance treatment (MMT).

Prospective, non-blinded, single-arm, 12-week intervention with standardized assessments.

Academic research laboratory affiliated with an on-site MMT clinic.

Fifteen (11 female) MMT patients completed a virtual reality, therapist-guided meditative intervention that included breathing and relaxation exercises; sessions were scheduled twice weekly. Assessments included functional magnetic resonance imaging (fMRI) of pain neuromatrix activation and connectivity (pre- and post-intervention), saliva cortisol and C-reactive protein (CRP) at baseline and weeks 4, 8 and 12; and self-reported pain and affective symptoms before and after each intervention session.

After each intervention session (relative to pre-session), ratings of pain, opioid craving, anxiety and depression (but not anger) decreased. Saliva cortisol (but not CRP) levels decreased from pre- to post-session. From pre- to post-intervention fMRI assessments, pain task-related left postcentral gyrus (PCG) activation decreased. At baseline, PCG showed positive connectivity with other regions of the pain neuromatrix, but this pattern changed post-intervention.

These preliminary findings demonstrate feasibility, therapeutic promise, and brain basis of a meditative intervention for OUD patients undergoing MMT.

These preliminary findings demonstrate feasibility, therapeutic promise, and brain basis of a meditative intervention for OUD patients undergoing MMT.The city and its urban biome provides an extreme laboratory for studying fundamental biological questions and developing best practices for sustaining biodiverse and well-functioning ecological communities within anthropogenic built environments. We propose by studying urban organisms, urban biotic communities, the urban biome, and the interactions between the urban biome and peri-urban built and natural environments, we can (1) discover new ‘rules of life’ for the structure, function, interaction, and evolution of organisms;(2) use these discoveries to understand how novel emerging biotic communities affect and are affected by anthropogenic environmental changes in climate and other environmental factors; and (3) apply what we have learned to engage residents of the urban biome, and design cities that are more biologically diverse, are provided with more and better ecosystem services, and are more equitable and healthier places to live. The built environment of the urban biome is a place that reflects history, economics, technology, governance, culture, and values of the human residents; research on and applications of the rules of life in the urban biome can be used by all residents in making choices about the design of the cities where they live. Because inhabitants are directly invested in the environmental quality of their neighborhoods, research conducted in and about the urban environment provides a great opportunity to engage wide and diverse communities of people. Given the opportunity to engage a broad constituency – from basic researchers to teachers, civil engineers, landscape planners, and concerned citizens – studying the translation of the rules of life onto the urban environment will result in an integrative and cross-cutting set of questions and hypotheses, and will foster a dialogue among citizens about the focus of urban biome research and its application toward making more equitable, healthy, livable, sustainable, and biodiverse cities.

The objective of this study was to assess the impact of the COVID-19 pandemic on healthcare providers (HCPs) at personal and professional levels.

This was a cross-sectional descriptive study. It was conducted using an electronic format survey through Qualtrics Survey Software in English. The target participants were HCPs working in any healthcare setting across Iraq. The survey was distributed via two professional Facebook groups between 7 April and 7 May 2020. The survey items were adopted with modifications from three previous studies of Severe Acute Respiratory Syndrome (SARS) and Avian Influenza Outbreak. Kruskal-Wallis test was conducted to determine the difference in the pandemic impact according to the dealing with COVID-19 cases.

The authors received 430 surveys from HCPs representing 14 provinces. Approximately 60% of the participants were dealing with diagnosis or treatment of COVID-19 cases. More than 80% perceived high risk of infection and stress due to the COVID-19 pandemic. Additionally, 85.