Overexpression of SNORD72 could also stabilize ID2 mRNA and rescue the inhibitory effect of silencing lncRNA-LALR1. In conclusion, lncRNA-LALR1 is highly expressed in HCC and promotes tumor growth and invasion by upregulating SNORD72 to stabilize ID2 mRNA, implying that lncRNA-LALR1 might be a novel target for intervention of HCC.INTRODUCTION The incidence of mood disorders and psychopathology is more frequent in patients with epilepsy (PWE) than in the general population. Also, it has been reported that PWE suffer more seizures during certain phases of their menstrual cycle (MC). Still, limited information exists regarding the relationship between the physical and emotional changes during the MC in PWE. Therefore, in this study, we aimed to evaluate the mood and personality traits of PWE during their MC and to compare them with controls. METHODS A cohort of 22 PWE and nine controls was gathered. All the participants underwent psychiatric, electroencephalographic, and gynecological evaluations. RESULTS Overall, PWE scored higher in depression compared with controls (p  less then  0.05), PWE also obtained higher scores for the personality traits of neuroticism and self-isolation (p  less then  0.05). During the evaluation of the symptoms of premenstrual syndrome (PMS), PWE were more symptomatic during the early follicular (EF) phase in comparison with the rest of the phases of their MC, whereas the control group showed the known premenstrual pattern of symptoms during the late luteal (LL) phase. The frequency of seizures (40.6%) and electroencephalographic abnormalities (34.8%) was also higher during the EF phase of the MC when compared with the other phases of the MC (p  less then  0.05). CONCLUSIONS The results of this study suggest that unlike the PMS present in women without epilepsy, PWE appear to show a “menstrual syndrome” that consists of similar mood changes and physical symptoms. This arrangement of symptoms seems to have an impact on the increase in seizure activity. PURPOSE We investigated the rate of driving in patients with psychogenic nonepileptic seizures (PNES) from a large cohort in Iran. We hypothesized that these patients commonly do not drive. We also investigated the potential factors that may be associated with driving in these patients. METHODS In this retrospective study, all patients with PNES, who were diagnosed at Shiraz Comprehensive Epilepsy Center, Iran, from 2008 until 2019, were investigated. Age, gender, age at seizure onset, seizure semiology and characteristics, driving history reported by the patients, and video-electroencephalography recordings of all patients were registered routinely. RESULTS During the study period, 221 patients with PNES only had the inclusion criteria and were studied. Forty-eight patients (21.7%) reported that they were driving a car in their routine daily lives. Male sex (odds ratio 13.2; 95% confidence interval 4.98-35.45; p = 0.0001) and being employed (odds ratio 8.08; 95% confidence interval 3.16-20.69; p = 0.0001) were independently significantly associated with driving. CONCLUSION We observed that only about one-fifth of adult patients with PNES reported driving. see more It is important for the scientific community to develop practice guidelines on how to counsel patients and also the related authorities (e.g., department of motor vehicle authorities) on driving restrictions in people who are affected with PNES. Previous studies have reported that miners (and other workers) exposed to high levels of diesel engine exhaust (DEE) have an increased risk of lung function decline. The main objective of this study was to evaluate associations between exposure to different components associated with DEE in relation to lung function across a 12-h working shift. Eighty underground gold miners and twenty surface miners completed spirometry and questionnaires at the beginning and end of their 12 h work shift. Personal exposure to elemental carbon (EC), volatile organic compounds (VOCs), nitrogen dioxide (NO2), particle size and particle number were monitored during their shift. Multiple regression models were used to examine the associations between DEE and lung function, adjusting for a range of covariates. Underground miners were exposed to higher levels of EC, VOCs, NO2, and particle number and larger mean particle size than surface miners. Cross-shift reduction in Z-score value of FEV1/FVC in underground miners was statistically significantly greater than those of surface miners. The cross-shift change in Z-score value of FEV1/FVC was associated with exposure to higher concentration of EC and particle number, but not with VOCs, NO2 and particle size. Occupational exposure to diesel engine exhaust in current Australian gold mines is substantial. Exposures were higher in underground miners and had a negative association with their lung function over a single 12-h shift. Nanoparticles based drug delivery system offers an alternative strategy to overcome several therapeutic limitations of various human ailments, particularly in age-linked Alzheimer’s disease. Results of our previous cell-free studies indicated that α-bisabolol loaded solid lipid nanoparticles (ABS) significantly inhibited the aggregation of Aβ25-35. The present study intended to evaluate the neuroprotective effect of ABS against Aβ25-35 induced toxicity in Neuro-2a cell lines. The results of in vitro cell line study revealed that pretreatment of Neuro-2a cell lines with ABS (5 & 10 μg/ml) significantly suppressed the production of free radicals such as reactive oxygen species (ROS)/reactive nitrogen species (RNS), and also augmented the ROS mediated macromolecular damages and loss of mitochondrial membrane potential induced by toxic Aβ peptide when compared to the standard drug donepezil (50 μg/ml). Moreover, reduced β-secretase, caspase-3, and cholinesterase activities were observed in the cells pretreated with ABS, which clearly evidenced the neuroprotective effect of ABS. Reduced expression of Bax and induced expression of Bcl-2 proteins observed through western blot analysis and live/dead cell viability analysis of Neuro-2a cells through confocal microscope further validated that ABS protects the cells from Aβ induced apoptosis. Taken together, the outcome of the present study signifies the neuroprotective effect of ABS against the Aβ induced toxicity in in vitro model of Neuro-2a cells. Emissive lanthanide ions are considered as luminescent species or optical probes for the analytes in ambient conditions or even cellular environment. But a variety of factors determine the stability of photo-luminescent signals due to the excitation sources, statistical errors or the selection of solvents. In this way, ratiometric assays effectively circumvent such problem and lead to more sensitive and reliable results. Herein, an organic-inorganic hybrid material has been developed with the europium complex as the core and the terbium species can be attached onto the surface of silica host. As for the evaluation of the internal structure, the terbium complex has been integrated into the hybrid network via weak forces and its sensitizing moiety will be easily attacked by Lewis acid-base interactions. In a different manner, the europium complex has been shielded from perturbation due to the protection of the outside silica shells. Such sharp difference in response to fluoride induces drastic signal changes and the conversion of binding process between fluoride and molecular receptors (boronic group) into readable optical outputs has afforded the lower detection limit for F- as 0.17 μM. In addition, the intracellular uptake and distribution of the hybrid material have been explored. V.Quetiapine (QUT) is an atypical antipsychotic drug indicated for the treatment of schizophrenia and acute manic episodes associated with bipolar disorders. A simple, rapid, and highly sensitive micellar spectrofluorometric method has been developed and validated for quantitation of QUT in its pharmaceutical formulations with application to content uniformity test, in presence of its degradation product and in rat plasma. The proposed method was based on the enhancement of the fluorescence intensity of QUT in 2% v/v tween 80 micellar solution. The fluorescence intensity was measured at 372 nm after excitation at 261 nm. A linear relationship was achieved between the fluorescence intensity and the drug concentration in the range of 20-1000 ng/mL with 18.5 and 6.3 ng/mL as limits of quantitation and detection, respectively. The proposed method was extended to study the stability of QUT after its exposure to different forced degradation conditions such as; acidic, alkaline, oxidative, photolytic and thermal conditions according to ICH guidelines. The study revealed that QUT is stable under all the of these conditions except the oxidative one. Furthermore, the high sensitivity of the micellar method permits its application for determination of QUT in rat plasma with good percentage recovery as well as determination of Cmax. V.On account of the recalcitrant and highly toxicity of organonitrile substrates, traditional processes are limited by HCN poisoning thus inefficient. This article proposed a novel anaerobic fluidized bed reactor with electric field activation (AFBR-EFA) which had a 260-day continuous operation. The operation aims to explore the practicability of the enhanced reduction of isophthalonitrile (IPN), with emphasis on the optimum operation parameters and synergistic effect between electric field and anaerobic processes. The results showed that relatively higher voltage (1.0 V  less then  V  less then  1.6 V) had a positive impact on reduction enhancement. High removal could be obtained at high initial concentration, low methanol dosage and short HRT which indicated that tolerance to shock loading was significantly enhanced in AFBR-EFA. Furthermore, EFA visibly motivated the enrichment of electrochemically active bacteria and various autotrophic IPN degradation-related species. The significantly efficient performance makes the potential for full-scale application of the AFBR-EFA markedly improved, particularly for treating hard-biodegraded contaminants. Gastrointestinal nematode (GIN) infections in cattle cause appetite suppression which leads to poor feed conversion, reduced weight gain and reduced milk production. Overuse and exclusive reliance on anthelmintic drugs has resulted in widespread resistance in many parasitic nematode species infecting livestock making control increasingly difficult. Wild ruminants are competent hosts of a number of nematode species that typically infect and are best adapted for cattle, sheep, and goats. Thus, the potential exists for wild ruminants to act as reservoirs in the translocation of domestic GIN, including those carrying anthelmintic resistance mutations as well as susceptible genotypes. The potential for parasite exchange is heightened by interfaces or ecotones between managed and wild rangelands, and by perturbations linked to climate warming that can increasingly alter the distributions of wild ungulates and their interactions with domestic and free-ranging ruminants. To investigate the extent to which wild ruminants harbour parasites capable of infecting domestic ruminants we first performed an epidemiological study of feces from wildlife hosts that spanned 16 states and included white-tailed deer (85 % of the samples), pronghorn, elk, mule deer, bighorn sheep, moose, cattle, and caribou across the United States.